So far my experience with asymbiotic seed sowing has been appallingly poor. Out of the several dozen or so flasks I have sowed I have only had success with three flasks, all Anacamptis laxiflora. While a few have obviously succumbed to bacterial and fungal infection and a few froze in the fridge (I have a better understanding of the really cold spots in my fridge now!) the main problem has simply been zero germination. I suspect that the error lies in the time taken when they are being sterilised, or when they are being sown (which tends to incorporate some liquid).
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